Preprint · under construction
Circadian resonance in Cannabis sativa. Yield, morphology and resin under four periods with paired clones.
E. I. Vitor · Supercannabis Team · Supercycler network · and whoever joins
Abstract
A light and dark cycle that doesn't add up to 24 hours breaks the coupling between day and night, and leaves two variables where there used to be one. This trial tests whether the endogenous period of Cannabis sativa sits close to 27 hours and whether, because of that, a cycle of 13 hours of light and 14 of dark yields more than one of 12 and 12 while receiving less total light. Four cubicles run periods of 24, 26, 27 and 28 hours in parallel, with four cloned genetics repeated in each one, and one container of chrysanthemums per cubicle as a second species. The primary outcome is dry flower weight per plant on day 70 from the flip, with a simultaneous cut in all four. The predictions were published before the setup and are not edited. The 28 hour arm is there so that we can be wrong in public.
Keywords. Plant chronobiology, circadian resonance, T period, photoperiod, Cannabis sativa, supercycle, pre-registration, open science.
1. Where it comes from closed
Circadian resonance has been described since 1972 in flies and since 2005 in plants, and in 2026 it was taken to production in lettuce with 29% more biomass without adding a single photon. What we could not find published is anyone who has run it on a flowering crop, measuring flower and secondary metabolites instead of leaf.
On the practical side, there are more than a hundred rooms in the network running 26 and 27 hour cycles and reporting the same pattern. None of that is controlled. This trial exists to put a control next to it.
2. Hypothesis and predictions closed
That the clock's own period in Cannabis sativa sits close to 27 hours, and that an external cycle of that length matches the plant's internal machinery better than the 24 hour day we subject it to out of habit.
The five predictions were published dated 16 August 2026, before the setup and with no data taken, and they appear in full further up on this same page. They are never edited, not even to fix a comma. If the data breaks them, they get published broken.
3. Materials closed
Four genetics, one plant of each in every arm, and the same clone across all four. Genetic works as a block, so the replication unit is the genotype.
| Genetic | Declared flowering | Provenance and notes |
|---|---|---|
| Sweet 16 Lit Farms | ±63 d | Project 4516 × Grandi Candy, mostly indica. Breeder entry on SeedFinder. It is the only one of the three with a published, verified time. |
| Lemon Cherry Fire F2 Tiki Seeds | unpublished | (Lemon Cherry Gelato × Zerbert) F2. The breeder publishes lineage but no flowering time. Lemon Cherry Gelato runs around 56 to 65 days depending on the bank; being an F2 it segregates, so we expect real spread between plants rather than a single number. |
| SC-G01 n.n. | unknown | Indica-looking plant, nomen nescio. Donated clone, received as “Mack #2”, lineage unverified. It is listed by accession code and not by the name it came with, which is not confirmed. |
| Hell-echo Berryfreak × Freakshow | unpublished | Berryfreak × Freakshow. The breeder publishes lineage but no flowering time. Freakshow is the one that brings the atypical leaf, so this genetic already has an odd morphology before we touch its photoperiod. Here we look at yield and resin, and leaf shape does not count as an effect of the regime. |
A second species, one container per arm
Each of the four cubicles also holds a container of chrysanthemums, one per regime. The chrysanthemum is the other textbook short-day plant, with photoperiodic flowering described decades ago, so it is the cheapest way to ask whether this is about the photoperiod or about the species we have been looking at.
It does not enter the primary endpoint or any prediction. It is a declared observation, with no replication inside each arm. We log flower-bud date, opening date and appearance, with a photo. If the ordering across arms resembles the one above it is a lead for the next trial; if it does not, it refutes nothing that is predicted here.
4. Procedure closed
The cut criterion, drying, and exclusions
All four arms are cut on the same calendar day, day 70 from the flip. There is no per-plant maturity criterion, because under these regimes maturity does not exist as a state. Day 70 comes from the flowering time of the slowest known genetic (Sweet 16, ±63 d) plus margin for an F2 that segregates and for a genetic of unknown timing. Dry weight plateaus rather than falling, so that margin does not affect the primary endpoint.
With a single date for all four, the long-period arms get no extra days and the curve in prediction 1 stays comparable. With a different cut rule per arm it would stop being so.
Drying. Wine fridge at 14 °C and 65% relative humidity, all plants in the same space. Each one is weighed when the flower moisture meter reads 12%, with the same meter and the same scale, at 0.1 g resolution. Every reading is logged. A moisture point rather than fixed days, because it removes how much water each plant was carrying from the equation.
Exclusions. A plant that hermaphrodites or dies is excluded from the primary endpoint and reported with its arm, its genetic and the day. It is not replaced. If the events concentrate in one arm, that is a result for that arm.
What we measure
| What | How |
|---|---|
| Primary endpoint | Dry flower weight per plant. Chosen in advance. Wet weight is not used to conclude anything. |
| Light | Absolute DLI is not measured and is not claimed. We have no PAR sensor. What is determined is the light ratio between arms, because the four fixtures are the same model, at the same power, at the same height and over the same area, and with matched fixtures, the DLI ratio is the hours ratio. The hours actually executed are verified against the light action log, which records the expected and the confirmed time of every transition, so they are the hours that happened and not the hours on the schedule. Parity between cubicles is checked with a lux reading at a fixed point in each, at setup and at cut. With identical spectra, the lux ratio is the PPFD ratio. |
| Environment | Temperature, humidity and VPD logged continuously by Supercycler with Tuya sensors, per cubicle, for the whole trial. |
| Morphology | Photos with a scale in frame and growth mapping with the Eyes cameras. Flowering sites, calyx length and stigmas per flower. |
| Calendar | Days from flip to cut, fixed in advance at 70 for all four arms. |
| Design | Blocked by genetic. The same clone goes into all four arms. One cubicle per arm, four plants per cubicle, one of each genetic, plus one container of chrysanthemums per arm which sits outside the primary endpoint. |
| Room and light | Four 1×1 m indoor tents, 300 W Sylvania each, switched by Shelly from Supercycler. Real schedule adherence is verified against the light action log, which records the expected and the confirmed time of every transition. |
| Irrigation and substrate | Hunter drip, two 2 L/h emitters per pot, 10 L pots with Growers Supersoil. Irrigation is attached to the cycle, same amount and same relative hour. Actual counts and volumes per arm are reported, measured rather than estimated. |
| Feeding | Short flowering schedule, with the stage indexed to the cycle and not to the calendar, like everything else. |
| Chemistry | THC and CBD series with a Purpl Pro from the declared week, taken as a relative trend and not as absolute potency, because the method is NIR and it is validated on dried, ground sample. The number we publish comes from the CONICET laboratory. Samples are pre-declared now, upper third of the apical cola, same mass, one plant per genetic per arm, taken at cut. |
5. Planned analysis open
This is where the document is incomplete, and we'd rather say so than paper over it. There is one plant per genetic and per arm, so there is no average with its difference of means test that holds up. What holds the comparison together is that the genetic works as a block and the clone is the same one in all four cubicles.
What we have in mind is to look at the order of the four arms inside each genetic. With four arms there are twenty four possible orderings and only one of them is what the hypothesis predicts, so the probability of hitting it by chance in all four genetics at once can be computed exactly and it is small. It is a permutation test of minimum size. We know it is arguable, and that's why it sits here and not in a footnote.
- Is the statistic the order of the four arms, the distance between the best one and the control, or the fit of a curve with a maximum?
- Is dry weight analysed raw or normalised within each genetic, and in that case what is it normalised against?
- How does the chrysanthemum come in, if it comes in at all, or does it stay only as an observation with a photo?
All three get answered before there is a single dry weight on the scale. Choosing the analysis after seeing the data is the most common and most elegant way of convincing yourself, and the date on this section is the only proof that we didn't do it.
This gets decided with whoever wants in. If you work with block designs, or this is your field, write to us through the form at the foot. Whatever gets agreed goes into the document with a date and a name.
6. What would count as refutation closed
That 14/14 matches or beats 13/14 on dry weight. That breaks the peak, and with the peak the resonance explanation falls.
That the direction of the effect is not consistent across genetics. If one goes up and another goes down, what we have is noise and not an effect.
That 12/12 ties with 13/14 on dry weight. With no difference there is nothing to explain.
The results are published on this page in all three cases.
7. Authorship and document status
The author list is not closed. Anyone who contributes method that makes it into the document, or runs a declared replication with their own room and their own genetics, is listed as a collaborator the day this gets published. You don't have to ask permission to start, you have to let us know.
This document changes. Every version is logged in the change log at the end of the page, with the date and with what it said before in plain sight.